G. Giczey et al., HOMOLOGOUS TRANSFORMATION OF TRICHODERMA-HAMATUM WITH AN ENDOCHITINASE ENCODING GENE, RESULTING IN INCREASED LEVELS OF CHITINASE ACTIVITY, FEMS microbiology letters, 165(2), 1998, pp. 247-252
A 42-kDa endochitinase encoding gene, Tham-ch, was cloned by screening
the genomic library of Trichoderma hamatum strain Tam-61 with a PCR-a
mplified chitinase sequence from the same fungus. Tham-ch with its own
regulatory sequences was reintroduced into the host strain. The integ
ration of the transforming construct was stable only in one copy. Homo
logous integration occurred in nine transformants, while non-homologou
s integration was detected in one transformant. All but one transforma
nt expressed higher levels of chitinase activity in comparison to the
wild-type recipient strain; the maximum level of increase was 5-fold.
Duplicating the copy number of the highly conserved similar to 42-kDa
endochitinase encoding gene appears to be one potential means by which
the biocontrol capability of the Trichoderma mn species might be impr
oved. (C) 1998 Federation of European Microbiological Societies. Publi
shed by Elsevier Science B.V. All rights reserved.