DEVELOPMENT OF AN INTEGRATIVE DNA TRANSFORMATION SYSTEM FOR THE YEASTCANDIDA-UTILIS

We report here the development of an auxotrophic transformation system for the food yeast Candida utilis. To facilitate molecular studies in Candida utilis, we isolated auxotrophic strains for uracil biosynthes is by the combination of NTG-mutagenesis and 5-fluorotic acid (FOA) se lection. The ura(-) mutation could be functionally complemented by the homologous URA3 gene. We used both, LiAc and electroporation methods to direct insertions at the ura3 locus through homologous recombinatio n. (C) 1998 Published by Elsevier Science B.V. All rights reserved.