A CONVENIENT METHOD FOR AFFINITY PURIFICATION OF MALTOSE-BINDING PROTEIN FUSIONS

Maltose binding protein of Escherichia coli is frequently employed as a fusion partner for the biosynthesis of polypeptides and proteins, la rgely because of the advantage provided by affinity purification of su ch a fusion. A mixture of cellulose and starch is shown to be a simple and effective alternative to the use of other, more complicated media which are often used for affinity chromatography of maltose binding p rotein fusions. (C) 1998 Elsevier Science B.V. Al rights reserved.