BCL-2 GENE AND ITS FAMILY GENES BAX, BCL-XL AS WELL AS FAS APO-1 AND THEIR CLINICAL-SIGNIFICANCE IN ACUTE-LEUKEMIA/

Objective To study the suppressing genes of apoptosis, namely Bcl-2, i ts family genes Bar, Bcl-XI, and the inducing gene of apoptosis Fas/Ap o-1. Methods The techniques of cytoimmuno-histiochemical stains, Weste rn blotting and Northern blotting were used. Results It was found that the antigens of Bcl-2 in acute myelogenous leukemia (AML) and acute l ymphocytic (ALL) was higher than that in the normal (P < 0.01). At sam e time, Bcl-2 was obviously lower expression in complete remission (CR ) group than that in non-remission (NR) one by retrospective analysis (P < 0.01). Though Bcl-2 was low expression in CR with Western blottin g, there was no statistical significance (P > 0.05). In CR group the e xpression of Bcl-2 mRNA was obviously lower than that of NR one (P < 0 .01). Even though leukemia expression of Bar with cytoimmuno-histioche mical stain was also lower than that in the normal people, there was n o difference between CR and NR with cytoimmuno-histiochemical, Western blotting and Northern blotting (P > 0.05). There was difference of Bc l-XI mRNA in two groups (P < 0.01). The expression Fas/Apo-1 in leukem ia was lower than that in normal people (P < 0.01). But in CR and NR, there was no difference with cytoimmuno-histiochemical stain and Weste rn blotting. Conclusions The Changes of genes and their proteins are s ignificant theoretically and clinically. The antigen expression of Bcl -2 and the expression of Bcl-2 mRNA may be considered as a prognostic index for AML.