COMPUTER-ASSISTED SPERM MOTION ANALYSIS OF BOVINE SPERM TREATED WITH INSULIN-LIKE-GROWTH-FACTOR-I AND INSULIN-LIKE-GROWTH-FACTOR-II - IMPLICATIONS AS MOTILITY REGULATORS AND CHEMOKINETIC FACTORS

The effects of insulin-like growth factors (IGFs) I and II on motility of bovine sperm were examined using a computer-assisted sperm motion analyzer (CASA). The following kinematic parameters were examined: per centage of rapidly moving cells, straight-line velocity, curvilinear v elocity, average path velocity, amplitude of lateral head displacement , and beat cross frequency. Sperm were treated with IGF-I (100 ng/mL) or IGF-II (250 ng/mL) and compared to sperm in modified Tyrodes' mediu m only (control) at 90, 180, and 360 min using CASA. Insulin-like grow th factor I and II increased the percentage of rapidly moving cells, s traight-line velocity, curvilinear velocity, average path velocity, am plitude of lateral head displacement, and beat cross frequency compare d to the control treatment. These results indicate that IGFs may be in volved in initiation and maintenance of bovine sperm motility.