Many drugs act as sperm stimulants and are of clinical value for male
infertility. Current research deals with the physiological mechanisms
of sperm motility/sperm stimulation and how long the effect lasts. For
such a study, long-term monitoring of sperm motility becomes essentia
l for traditional semen evaluation. A new system was designed to deal
with the microscopic images of semen. Its performance was evaluated by
studying the effect of EDTA on sperm motility. EDTA increased sperm c
urvilinear velocity (V-cl) and straight-line velocity (V-sl) by 31 and
20%. EDTA also prolonged the duration of motility by 68 and 61%, resp
ectively. However, EDTA had less effect on the linearity of forward pr
ogression (L-in). The proposed system can analyze semen and does well
at monitoring sperm motility for short term and long term. It may be v
aluable to test the possible role of sperm stimulation for male infert
ility and assisted reproduction.