IDENTIFICATION OF HYALURONAN-BINDING PROTEINS USING A BIOTINYLATED HYALURONAN PROBE

Authors
HOARE K SAVANI RC WANG C YANG B TURLEY EA
Citation
K. Hoare et al., IDENTIFICATION OF HYALURONAN-BINDING PROTEINS USING A BIOTINYLATED HYALURONAN PROBE, Connective tissue research, 30(2), 1993, pp. 117-126
Citations number
26
Categorie Soggetti
Cytology & Histology
Journal title
Connective tissue research → ACNP
ISSN journal
03008207
Volume
30
Issue
2
Year of publication
1993
Pages
117 - 126
Database
ISI
SICI code
0300-8207(1993)30:2<117:IOHPUA>2.0.ZU;2-9
Abstract
A method for detecting hyaluronan (HA)-binding proteins in transblot a ssays using biotinylated HA (BHA) is described. Some of the binding ch aracteristics of a novel HA receptor termed RHAMM (Receptor for HA-Med iated Motility) are characterized using this assay. The method is also used to detect other HA-binding proteins in tissue homogenates. This method is semiquantitative, rapid, reproducible, sensitive and therefo re of potential use in identifying the levels of HA-binding proteins i n different cells and tissues.