WOUND-HEALING OF INTESTINAL ANASTOMOSIS AFTER DIGESTIVE SURGERY UNDERSEPTIC CONDITIONS - PARTICIPATION OF LOCAL INTERLEUKIN-6 EXPRESSION

This study aimed to evaluate the integrity of anastomotic wound healin g after digestive surgery under septic conditions and to observe local interleukin-6 (IL-6) expression around the anastomotic segment. Exper imental animals mere separated into lipopolysaccharide (LPS) and contr ol groups. Each was injected with LPS or normal saline solution into t he peritoneal cavity 24 hours before transection and anastomosis of th e colon. The anastomotic bursting pressure (ABP) and tissue hydroxypro line concentration (HP) were measured as indicators of wound healing. Immunohistochemical staining for IL-6 was performed on tissue samples obtained from the anastomotic segment, lung, liver, and kidney. The re active cells were counted by light microscopy. The ABP and HP were sig nificantly lower in the LPS group than the control group 7 days after the surgery. In the LPS group, IL-6 expression around the anastomotic segment was enhanced I and 6 hours after surgery but suppressed 24 hou rs afterward. In contrast, IL-6 expression in Lung, liver, and kidney was enhanced in the LPS group 24 hours after surgery but not in the co ntrol group. It is suggested that anastomotic wound healing is impaire d after digestive tract surgery under septic conditions, and local IL- 6 expression participates in wound healing.