LIQUID, CHROMATOGRAPHY DIRECTLY JOINTLY COMBINED WITH NUCLEAR-MAGNETIC-RESONANCE SPECTROSCOPY AND MASS-SPECTROMETRY

The hyphenation of high performance liquid chromatography (HPLC) with sensitive and information-rich spectroscopic detection techniques such as mass spectrometry (MS) and nuclear magnetic resonance spectroscopy (NMR) offers the potential of rapid and highly efficient methods for the characterization and identification of unknowns in complex matrice s. Although the coupling of HPLC with MS is widely known and appreciat ed, the benefits of HPLC-NMR are only now becoming apparent. This arti cle discusses the advantages of using both NMR and MS coupled simultan eously in the same HPLC separation. It also includes examples from the field of drug metabolism analysis.