ACCUMULATION AND EFFECTS OF BENZO(A)PYRENE ON CYTOCHROME-P450 1A IN WATERBORNE EXPOSED AND INTRAPERITONEAL INJECTED JUVENILE TURBOT (SCOPHTHALMUS-MAXIMUS)

Juvenile turbot (Scophthalmus maximus) were exposed to benzo(a)pyrene (BaP) for 14 d using a glass bead generator flow-through system. Expos ure was followed by a recovery period of 16d. The highest BaP concentr ation ill the edible portion of the fish, 16.5 +/- 4.3 mu g BaP/kg, wa s observed on the first day. Then concentrations dropped following fir st-order kinetics. BaP was below detection level at the end of the exp eriment. A statistically significant increase in bile fluorescence was observed from day 9 until the end of the experiment, suggesting the e limination of BaP metabolites by this route. No significant difference s between control and exposed fish in EROD activity and CYP1A concentr ation, measured by immunodetection method, were observed. Intraperiton eal injection of 2.5 mg BaP/kg in juvenile turbot induced EROD activit y. Under waterborne experimental conditions, bile fluorescence was obs erved to be a more sensitive biomarker of BaP exposure than EROD activ ity and CYP1A measurement. (C) 1998 Elsevier Science Ltd. All rights r eserved.