MONOCLONAL AND POLYCLONAL ANTIBODIES AGAINST FISH VITELLOGENIN FOR USE IN POLLUTION MONITORING

Two monoclonal antibodies (KB-1 and BN-5) and one polyclonal antibody (AA-1) were prepared against purified vitellogenin (Vtg) from Atlantic salmon (Salmo salar), and tested for their ability to bind Vtg from p lasma of various other fish species. The MAb KB-1 was found to bind sp ecifically to Vtg from the Salmo species, Atlantic salmon (Salmo salar ) and brown trout (Salmo trutta). The MAb BN-5 showed a much wider cro ss-reactivity, binding to Vtg from both Salmoniformes species (Atlanti c salmon, brown trout, rainbow trout and Arctic charr) and Pleuronecti formes species (turbot and halibut). The widest cross-reactivity Iras observed with the polyclonal antibody AA-1 which, in addition to the s pecies recognized by the MAb BN-5, also bound to Vtg from Atlantic cod (Gadiformes). (C) 1998 Elsevier Science Ltd. All rights reserved.