VARIATION IN LEVELS OF CYTOCHROME P4501A, 2B, 2E, 3A AND 4A-IMMUNOPOSITIVE PROTEINS IN DIGESTIVE GLAND OF INDIGENOUS AND TRANSPLANTED MUSSEL MYTILUS-GALLOPROVINCIALIS IN VENICE LAGOON, ITALY

Mytilus galloprovincialis digestive gland microsomes were prepared fro m (i) indigenous populations sampled from a clean reference (Lido) and an urban contaminated site (Salute) and (ii) mussels transplanted for up to 3 weeks from Lido to an industrial-contaminated site (CVE) in V enice Lagoon, Italy. Western, blot analysis was performed using antibo dies to Jive mammalian or fish CYP forms (IA, 2B, 2E, 3A, 4A). Simulta neously run M.. edulis digestive gland partially purified CYP aided id entification of immunopositive bands. Levels of CYP1A, CYP2E, and CYP4 A-immunopositive proteins were 50 to 300% higher in indigenous M. gall oprovincialis front Salute compared to Lido (p < 0.05). Three weeks af ter transplantation to CVE, levels of only the CYP1A-immunopositive pr otein were determined to be higher (63%) than levels for Lido (p<0.05) , indicating that anti-CYP1A shows greater specificity,for a contamina nt-inducible CYP form than the of her antibodies. (C) 1998 Elsevier Sc ience Ltd. All rights reserved.