DNA-ADDUCTS IN PERCH, PERCA-FLUVIATILIS, FROM A CREOSOTE-CONTAMINATEDSITE AND IN PERCH EXPOSED TO AN ORGANIC-SOLVENT EXTRACT OF CREOSOTE-CONTAMINATED SEDIMENT

DNA adducts were analysed by P-32-postlabelling in the liver of wild p erch, Perca fluviatilis, from a site ill the river Angemanalven, Swede n, whose sediments ave contaminated with creosote. The level of DNA ad dicts in fish from the creosote contaminated site was 6.82 +/- 4.08 nm ol mol(-1) nucleotides compared to 0.21 +/- 0.20 nmol mol(-1) nucleoti des in fish from a reference site. Two diagonal zones including 10-12 adduct spots were observed on autoradiograms derived from perch front the contaminated site, while only very faint adduct,spots were observe d on autoradiograms derived from the reference site. In the laboratory , groups of perch were exposed to an organic solvent extract Prey,and from the creosote contaminated sediment or to benzo(a)pyrene by oral a dministration, Perch treated with the extract had adduct patterns very similar to those observed in perch from the contaminated field site. One minor adduct was identified bq, co-chromatography,with a P-32-labe lled standard of 7R,8S,9S-trihydroxy, 10R (N(2)deoxyguanosl-3'-phospha te)- 7,8,9,10- tetrahydrobenzo (a)pyrene (BaPDE-dG-3'p). Autoradiogram s derived from the B(a)P exposed fish showed one major adduct and seve ral less intense spots. The major B(a) P-adduct had the same chromatog raphic properties as the standard BaPDE-dG-3'p-adduct. (C) 1998 Elsevi er Science Ltd. All rights reserved.