M. Reinecke et H. Segner, IMMUNOHISTOCHEMICAL LOCALIZATION OF CYTOCHROME P4501A IN DEVELOPING TURBOT, SCOPHTHALMUS-MAXIMUS, Marine environmental research, 46(1-5), 1998, pp. 487-491
In order to interpret toxic responses of fish embryos and larvae to xe
nobiotics, knowledge on biotransformation capacities of fish early lif
e stages is important. This study examines by means of immunohistochem
istry the expression of cytochrome P4501A (CYP1A), a major inducible e
nzyme of xenobiotic metabolism, in the organs of turbot, Scophthalmus
maximus, larvae. A polyclonal antiserum (BN-I, Biosense Laboratories),
prepared to conserved piscine CYP1A sequences, was used to stain the
enzyme in paraffin-embedded tissue sections. Strong immunoreactivity f
or CYP1A was obtained in liver parenchymal cells, but hepatic sinusoid
eal epithelia also responded positively. Extrahepatically, CYP1A immun
oreactivity was found in the kidney tubulus epithelium, in cardiac mus
cle cells, in the retina, as well as in endothelia of gill filaments,
glomerulum, heart, brain and muscles. The results indicate that the ti
ssue distribution patterns of CYP1A in larval turbot are comparable to
chose,found in the adults. (C) 1998 Elsevier Science Ltd. All rights
reserved.