CYTOSOLIC METAL SPECIATION STUDIES BY SEQUENTIAL, ONLINE, SE-IE HPLC-ICP-MS/

Authors
Citation
Az. Mason et Rf. Meraz, CYTOSOLIC METAL SPECIATION STUDIES BY SEQUENTIAL, ONLINE, SE-IE HPLC-ICP-MS/, Marine environmental research, 46(1-5), 1998, pp. 573-577
Citations number
7
Categorie Soggetti
Marine & Freshwater Biology","Environmental Sciences",Toxicology
ISSN journal
01411136
Volume
46
Issue
1-5
Year of publication
1998
Pages
573 - 577
Database
ISI
SICI code
0141-1136(1998)46:1-5<573:CMSSBS>2.0.ZU;2-A
Abstract
A procedure involving directly coupled HPLC-ICP-.MS is described for t he quantification of metals associated with cytosolic proteins, Select ivity is achieved by sequential fractionation bq size exclusion (TSK S W2000) followed by ion exchange chromatography (Showdex DEAE). Spectra on up to II masses for six elements were acquired simultaneously by s canning the quadrapole in the peak hopping mode. A flow injection loop inserted downstream of the columns was used to monitor analyte recove ry and quantify the ion intensity profiles from the MS. Reproducibilit y of analysis was approximately 2-5% and absolute detection limits wer e typically between 10 and 60 pg of analyte. The utility, of the techn ique for: (i) detecting abnormal distributions in cytosolic metals die to metal exposure; and (ii) determining the biological turnover of Cu and other metals associated with proteins is demonstrated using the m arine shellfish Littorina littorea exposed to elevated concentrations of Cd and Cu-65. (C) 1998 Elsevier Science Ltd. All rights reserved.