ACUTE HYPERINSULINEMIA FAILS TO CHANGE GLUT-4 CONTENT IN CRUDE MEMBRANES FROM GOAT SKELETAL-MUSCLES AND ADIPOSE-TISSUE

The effect of insulin on GLUT-4 protein level in samples of adipose ti ssue and skeletal muscles from goats was studied in vivo using an eugl ycemic hyperinsulinemic clamp. The clamp was maintained in conscious g oats for 6 h in the presence of amino acids to prevent insulin-induced hypoaminoacidemia. GLUT-4 protein was assessed in crude membrane prep arations from adipose tissue and four skeletal muscles (longissimus do rsi, tensor fasciae latae, anconeus and diaphragm) by Western blot ana lysis. No changes of GLUT-4 protein content were detected after 6 h of hyperinsulinemia in either adipose tissue or skeletal muscles from go ats. These results suggest that insulin is not the prime factor involv ed in the short-term regulation of GLUT-4 protein transporter content in insulin-sensitive tissues from goats. (C) 1998 Elsevier Science Inc . All rights reserved.