IN-VIVO P-31-NMR SPECTROSCOPY AND RESPIRATION MEASUREMENTS OF ANESTHETIZED GOBY (POMATOSCHISTUS SP.) PREEXPOSED TO AMMONIA

The effects of ammonia pre-exposure on high energy-phosphates and the intermediary metabolism of the marine teleost Goby (Pomatoschistus sp. ) were studied by in vivo P-31-NMR with simultaneous respirometry. No significant effect of ammonia pre-exposure was observed on oxygen upta ke, muscle intracellular pH or high-energy phosphate metabolism. Brief metomidate anaesthesia (10 mg l(-1)) followed by continuous light ana esthesia (1 mg l(-1)) was used to prevent excessive swimming. This had no effect on oxygen uptake, muscle intracellular pH or high-energy ph osphate metabolism. (C) 1998 Elsevier Science Inc. All rights reserved .