ROLE OF CD86 (B7-2) IN TRIGGERING OF ANTIGEN-SPECIFIC IGE ANTIBODY-RESPONSE BY LIPOPOLYSACCHARIDE

The role of CD86 in triggering of ascaris extract-specific IgE antibod y response by lipopolysaccharide was studied. The simultaneous adminis tration of anti-CD86 antibody with ascaris extract and lipopolysacchar ide prevented the production of IgE antibody response to ascaris extra ct. CD86(+) cells were detected in peritoneal cavities and spleens of mice injected intraperitoneally with lipopolysaccharide. CD86(+) cells appeared in peritoneal cavities and spleens eight hours after lipopol ysaccharide injection, and they were detectable for a week. CD86(+) ce lls in peritoneal cavities and spleens were mainly surface Ig-positive B-cells and some Ig-negative cells, It was suggested that lipopolysac charide induced the expression of CD86 mainly on B-cells, and that CD8 6(+) cells induced by lipopolysaccharide injection might play an impor tant role as antigen-presenting cells on triggering of ascaris extract -specific IgE antibody response by lipopolysaccharide. (C) 1998 Federa tion of European Microbiological Societies. Published by Elsevier Scie nce B.V. All rights reserved.