IL-13 INCREASES THE CPLA2 GENE AND PROTEIN EXPRESSION AND THE MOBILIZATION OF ARACHIDONIC-ACID DURING AN INFLAMMATORY PROCESS IN MOUSE PERITONEAL-MACROPHAGES

Pretreatment of mouse peritoneal macrophages with interleukin-13 (IL-1 3) potentiates the mobilization of arachidonic acid (AA) and the produ ction of HETEs but does not affect the production of cyclooxygenase me tabolites triggered by the suboptimal concentration of an inflammatory agonist (opsonized-zymosan), Cycloheximide suppresses these effects o f IL-13 suggesting that de novo protein synthesis is involved. Indeed, IL-13 induces a time-dependent increase in the levels of cytolosic PL A2 (cPLA2) protein and mRNA. This study demonstrates a new pathway for IL-13 to modulate the inflammatory process in macrophages via modific ations of cPLA2 expression and subsequent AA mobilization. (C) 1998 El sevier Science B.V. All rights reserved.