LINKAGE MAP OF ESCHERICHIA-COLI K-12, EDITION 10 - THE PHYSICAL MAP

A physical map, EcoMap10, of the now completely sequenced Escherichia coli chromosome is presented. Calculated genomic positions for the eig ht restriction enzymes BamHI, HindIII, EcoRI, EcoRV, BglI; KpnI, PstI and PvuII are depicted Both sequenced and unsequenced Kohara/Isono min iset clones are aligned to this calculated restriction map. DNA sequen ce searches identify the precise locations of insertion sequence eleme nts and repetitive extragenic palindrome clusters. EcoGene10, a revise d set of genes and functionally uncharacterized open reading frames (O RFs), is also depicted on EcoMap10. The complete set of unnamed ORFs i n EcoGene10 are assigned provisional names beginning with the letter ' 'y'' by using a systematic nomenclature.