D-GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE - STRUCTURAL BASIS AND FUNCTIONAL-ROLE OF THE ACYL TRANSFER-REACTIONS

The catalytic mechanism of D-glyceraldehyde-3-phosphate dehydrogenase is considered in the light of the available structural information. Th e design features of the enzyme molecule determining the pathway of th e acyl transfer, i.e., the transfer of the acyl group produced in the oxidative step of the reaction to one of the two accepters, inorganic phosphate or water, are discussed. The properties of enzyme forms poss essing cysteine residues oxidized to sulfenic acid derivatives are des cribed. The participation of these residues in the acyl transfer to wa ter is considered.