COMPOSITION OF ENDOGENOUS ALLELES CAN INFLUENCE THE LEVEL OF ANTISENSE INHIBITION OF GRANULE-BOUND STARCH SYNTHASE GENE-EXPRESSION IN TETRAPLOID POTATO PLANTS

The T-DNA composition was analysed of twelve potato genotypes obtained after transforming a tetraploid cultivar with an antisense granule-bo und starch synthase (GBSSI) gene. In five transformants (labelled TB50 nos.) the antisense GBSSI gene was driven by the CaMV 35S promoter, w hile in the remaining seven (labelled TBK50 nos.) the GBSSI promoter w as used. In these twelve transformants the antisense effect on amylose production in potato tuber starch ranged from complete suppression to no discernible inhibition, and the number of T-DNA insertions ranged from one to at least fifteen. The antisense effect of individual T-DNA loci in progeny of these transformants was studied. Progeny containin g a single T-DNA showed no inhibition of GBSSI activity. Only multiple , Linked T-DNA insertions resulted in substantial antisense inhibition . T-DNA fragments present in duplex in selfed progeny resulted in a la rger antisense effect than that in the parent (which contained the T-D NA insertions in simplex). Furthermore, the antisense effects of some T-DNA-containing linkage groups were influenced by the composition of endogenous GBSSI alleles. For practical breeding this implies that (1) the efficiency of obtaining primary potato transformants showing comp lete inhibition of GBSSI gene expression by antisense RNA is genotype- dependent, and (2) many transformants have to be produced per genotype to be able to select plants with maximum suppression of GBSSI and a m inimum number of T-DNA loci.