FIBER-TYPE-DEPENDENT EXPRESSION OF ADENOVIRUS-MEDIATED TRANSGENE IN MOUSE SKELETAL-MUSCLE FIBERS

Authors
HAGIWARA Y ISHII A NONAKA I KIKUCHI T TAKEDA S
Citation
Y. Hagiwara et al., FIBER-TYPE-DEPENDENT EXPRESSION OF ADENOVIRUS-MEDIATED TRANSGENE IN MOUSE SKELETAL-MUSCLE FIBERS, Acta Neuropathologica, 96(3), 1998, pp. 228-232
Citations number
28
Categorie Soggetti
Neurosciences,"Clinical Neurology",Pathology
Journal title
Acta Neuropathologica → ACNP
ISSN journal
00016322
Volume
96
Issue
3
Year of publication
1998
Pages
228 - 232
Database
ISI
SICI code
0001-6322(1998)96:3<228:FEOATI>2.0.ZU;2-M
Abstract
We show the efficient transduction and expression of the lacZ gene in the skeletal muscle of adult C57BL/10ScSn mice after adenovirus-mediat ed gene transfer. Of the myofibers in the tibialis anterior muscle 62% were beta-galactosidase positive after injection of the lacZ gene und er the control of the chicken beta-actin promoter and the cytomegalovi rus enhancer. The transduced gene was preferentially expressed in type IIA and IIX fibers, which were richer in oxidative enzymes than type LIE fibers.