TAXOIDS FROM CELL-CULTURES OF TAXUS-CHINENSIS

A cell culture of Taxus chinensis was found to produce taxoids in 4% y ield (dry weight) after stimulation with methyl jasmonate. From the cu lture medium and cell mass: 16 taxoids were isolated. The structures w ere determined by two-dimensional gradient-enhanced DQF-COSY, HMQC, HM BC, and NOESY NMR experiments. The major taxoids of the culture were e sterified at C-14 of the taxoid ring system. Five new taxoids were ide ntified as minor metabolites and were assigned as 2 alpha-benzoxy-4 al pha,10 beta-diacetoxy-1 beta,7 beta,9 alpha-trihydroxytax-11-ene, 2 al pha-benzoxy-4 alpha-acetoxy-1 beta,7 beta,10 beta-trihydroxy-9-dehydro tax-11-ene, 2 alpha-benzoxy-4 alpha,7 beta,9 alpha,10 beta-tetraacetox y-1 beta-hydroxytax-11-ene, 2 alpha,4 alpha,7 beta,9 alpha,10 beta-pen taacetoxytax-11-ene and 2 alpha,5 alpha,7 beta,9 alpha,10 beta-pentaac etoxy-4 beta,20-epoxytax-11-ene. (C) 1998 Elsevier Science Ltd. All ri ghts reserved.