Kv. Pervushin et al., SINGLE TRANSITION-TO-SINGLE TRANSITION POLARIZATION TRANSFER (ST2-PT)IN [N-15,H-1]-TROSY, Journal of biomolecular NMR, 12(2), 1998, pp. 345-348
This paper describes the use of single transition-to-single transition
polarization transfer (ST2-PT) in transverse relaxation-optimized spe
ctroscopy (TROSY), where it affords a root 2 sensitivity enhancement f
or kinetically stable amide N-15-H-1 groups in proteins. Additional, c
onventional improvements of [N-15,H-1]-TROSY include that signal loss
for kinetically labile N-15-H-1 groups due to saturation transfer from
the solvent water is suppressed with the 'water flip back' technique
and that the number of phase steps is reduced to two, which is attract
ive for the use of [N-15,H-1]-TROSY as an element in more complex NMR
schemes. Finally, we show that the impact of the inclusion of the N-15
steady-state magnetization (Pervushin et al., 1998) on the signal-to-
noise ratio achieved with [N-15,H-1]-TROSY exceeds by up to two-fold t
he gain expected from the gyromagnetic ratios of H-1 and N-15.