Wm. Jenq et al., ADHESION-PROMOTING PROPERTY OF LAMININ FROM NORMAL TISSUE AND FROM A TUMORIGENIC CELL-LINE, Connective tissue research, 30(1), 1993, pp. 59-73
The cell adhesion promoting activity of laminin isolated from normal h
uman placenta was compared with that isolated from mouse EHS tumor and
from the cultures of a mouse epithelial cell line B82 and its tumorig
enic derivative, B82HT The adhesion promoting properties of commercial
merosin isolated from placenta was also compared with the above prepa
rations using the human fibrosarcoma HT1080 cells. Percent attachment
was defined as (radioactivity extracted from attached cells)/(radioact
ivity in cells added to assay) x 100. HT1080 cells adhered more effici
ently on laminin (0.5 mug/well), isolated from the B82 rather than B82
HT cell conditioned medium, (82% vs 64%). Percent attachment of HT1080
cells on isolated native placental laminin or commercial merosin was
significantly higher compared to laminin from the EHS tumor (at 0.75 m
ug/well, 69%, 73% and 20% respectively). In parallel experiments the s
teady-state levels of mRNAs for subunits A, M, B1 and B2 in cultures o
f B82 and B82HT cells were determined. The ratio of mRNA for the lamin
in subunits in B82 and B82HT cells was 1:0.9 for the A chain, 1:0.6 fo
r the M chain, 1:0.4 for the B1 chain, and 1:0.3 for the B2 chain. Pro
tein studies indicated that the M subunit is absent in laminin prepara
tions from the EHS tumor whereas it is abundant in the laminin from pl
acenta and in commercial merosin. Laminin isolated from B82 cells cont
ains a higher proportion of the M subunit compared to that from B82HT
cells. The data suggest that there are functional differences between
the laminin found in normal tissue and that present in a solid tumor.
Functional differences were noted between the laminins synthesized by
the B82 cell line and its tumorigenic counterpart, B82HT. These differ
ences may result from the lack of gene expression for the laminin subu
nit M by the EHS tumor and by the lower degree of gene expression for
this subunit by B82HT cells. The possibility that the laminin synthesi
zed by the tumorigenic cell line may be structurally different from th
at synthesized by the B82 cells should also be considered.