OVEREXPRESSION OF CD44 - A USEFUL INDEPENDENT PREDICTOR OF PROGNOSIS IN PATIENTS WITH COLORECTAL CARCINOMAS

Background: The goal was to investigate the potential correlation betw een overexpression of CD44, high microvessel count (MVC), and p21(ras) with length of relapse-free and overall survival in patients with col orectal adenocarcinomas.Methods: CD44, factor VIII-related antigen (FV III-RA), and p21(ras) were localized immunohistochemically in patients with colorectal adenomatous polyps (n = 8) and adenocarcinomas (n = 9 8). The correlation between the expression of CD44, MVC in the areas w ith highest density, and p21(ras) with relapse-free and overall surviv al time was investigated. Data were analyzed statistically using univa riate and multivariate systems. Results: In patients with adenomatous polyps, the positivity of CD44, FVIII-RA, and p21(ras) was 75%, 62%, a nd 88%, respectively. In patients with colorectal carcinomas the posit ivity of CD44 was 55%, and for p21(ras) it was 52%. The median of FVII I-RA was 4 MVC (range, 0.0 to 32.33). MVC was greater than 4 in 53% of the patients with colorectal carcinomas. In univariate analysis, a si gnificantly longer relapse-free time (CD44: P = .0004; FVIII-RA: P = . 0006) and overall survival time (CD44: P = .0001; FVIII-RA: P = .001) were observed for patients with CD44-negative tumors and MVC below 4 a s compared to those with CD44-positive tumors and MVC greater than 4. Similar observations were noted in patients with Dukes B and C disease and the rectum as the site of tumor. In multivariate analysis, only C D44 con-elated significantly with both relapse-free (P = .0003) and ov erall survival (P = .00001). Conclusion: Univariate analysis showed CD 44 and MVC to be independent predictors of prognosis in colorectal car cinomas. Multivariate analysis showed that CD44 positivity was the mos t important indicator of an unfavorable prognosis for relapse-free and overall survival in patients with colorectal cancer. Thus, it can be deduced that whether CD34 is positive or negative in patients with col orectal cancer may have prognostic importance and in the future may be used as a factor in the pathologic evaluation of tumor specimens. Thi s hypothesis needs to be tested prospectively in a larger number of pa tients.