ENZYME FLOW MICROCALORIMETRY - A USEFUL TOOL FOR SCREENING OF IMMOBILIZED PENICILLIN-G ACYLASE

Screening of a representative series of immobilized penicillin G acyla se biocatalysts (enzyme, cells) using enzyme flow microcalorimetry is described. Immobilized penicillin G acylase biocatalysts were either p repared in the laboratory by various techniques or obtained from four commercial manufacturers. An industrial strain of Escherichia coli was entrapped in (poly)acrylamide gel or hardened calcium pectate gel. Se mi-purified enzyme was immobilized in various ways-either by covalent binding to oxirane-acrylic beads or chlorotriazine bead cellulose or b y entrapment in (poly)acrylamide gel. The validity of the enzyme flow microcalorimetry results was corroborated by a pi-I-stat method, showi ng enzyme flow microcalorimetry to be a suitable method for rapid scre ening of immobilized biocatalysts regardless of the immobilization tec hnique, carrier type or the biocatalyst source. (C) 1998 Society of Ch emical Industry.