TRANSFORMATION OF THE PHYTOPATHOGEN MYCOSPHAERELLA-GRAMINICOLA TO CARBENDAZIM AND HYGROMYCIN-B RESISTANCE

Citation
Ac. Payne et al., TRANSFORMATION OF THE PHYTOPATHOGEN MYCOSPHAERELLA-GRAMINICOLA TO CARBENDAZIM AND HYGROMYCIN-B RESISTANCE, Current genetics, 34(2), 1998, pp. 100-104
Citations number
23
Categorie Soggetti
Genetics & Heredity
Journal title
ISSN journal
01728083
Volume
34
Issue
2
Year of publication
1998
Pages
100 - 104
Database
ISI
SICI code
0172-8083(1998)34:2<100:TOTPMT>2.0.ZU;2-E
Abstract
The fungal wheat pathogen Mycosphaerella graminicola was transformed t o carbendazim and hygromycin B resistance. A beta-tubulin gene from a M. graminicola strain resistant to the fungicide carbendazim was clone d and used to transform a sensitive strain to carbendazim resistance. Hygromycin B-resistant transformants (up to 8 per mu g Of transforming DNA) arose at a higher rate than beta-tubulin transformants (up to 0. 6 per mu g of DNA). Transformants were able to infect wheat and were m orphologically similar to recipient strains.