IN-VIVO TRANSLATION OF THE TRIPLE GENE BLOCK OF POTATO-VIRUS-X REQUIRES 2 SUBGENOMIC MESSENGER-RNAS

Authors
VERCHOT J ANGELL SM BAULCOMBE DC
Citation
J. Verchot et al., IN-VIVO TRANSLATION OF THE TRIPLE GENE BLOCK OF POTATO-VIRUS-X REQUIRES 2 SUBGENOMIC MESSENGER-RNAS, Journal of virology, 72(10), 1998, pp. 8316-8320
Citations number
32
Categorie Soggetti
Virology
Journal title
Journal of virology → ACNP
ISSN journal
0022538X
Volume
72
Issue
10
Year of publication
1998
Pages
8316 - 8320
Database
ISI
SICI code
0022-538X(1998)72:10<8316:ITOTTG>2.0.ZU;2-A
Abstract
The 25-kilodalton (25K), 12K, and 8K movement proteins of potato virus X are derived from overlapping open reading frames (ORFs). Using an i n vivo complementation assay, we have shown that the 25K protein is ex pressed from a functionally monocistronic mRNA, whereas the 12K and 8K proteins are from a bicistronic mRNA. Translation of the 8K ORF is by leaky ribosome scanning through the 12K ORF.