MONITORING RETROVIRAL RNA DIMERIZATION IN-VIVO VIA HAMMERHEAD RIBOZYME CLEAVAGE

We have used a strategy for colocalization of Psi (Psi)-tethered riboz ymes and targets to demonstrate that Psi sequences are capable of spec ific interaction in the cytoplasm of both packaging and nonpackaging c ells. These results indicate that current in vitro dimerization models may have in vivo counterparts. The methodology used may be applied to further genetic analyses on Psi domain interactions in vivo.