THE MAJOR OPEN READING FRAME OF THE BETA-2.7 TRANSCRIPT OF HUMAN CYTOMEGALOVIRUS - IN-VITRO EXPRESSION OF A PROTEIN POSTTRANSCRIPTIONALLY REGULATED BY THE 5' REGION

Authors
BERGAMINI G RESCHKE M BATTISTA MC BOCCUNI MC CAMPANINI F RIPALTI A LANDINI MP
Citation
G. Bergamini et al., THE MAJOR OPEN READING FRAME OF THE BETA-2.7 TRANSCRIPT OF HUMAN CYTOMEGALOVIRUS - IN-VITRO EXPRESSION OF A PROTEIN POSTTRANSCRIPTIONALLY REGULATED BY THE 5' REGION, Journal of virology, 72(10), 1998, pp. 8425-8429
Citations number
40
Categorie Soggetti
Virology
Journal title
Journal of virology → ACNP
ISSN journal
0022538X
Volume
72
Issue
10
Year of publication
1998
Pages
8425 - 8429
Database
ISI
SICI code
0022-538X(1998)72:10<8425:TMORFO>2.0.ZU;2-D
Abstract
beta 2.7 is the major early transcript produced during human cytomegal ovirus infection. This abundantly expressed RNA is polysome associated , but no protein product has ever been detected. In this study, a stab le peptide of 24 kDa,vas produced in vitro from the major open reading frame (ORF), TRL4. Following transient transfection, the intracellula r localization was nucleolar and the expression was posttranscriptiona lly inhibited by the 5' sequence of the transcript, which harbors two short upstream ORFs.