COMPARISON OF SYNTHESIS FOR DETERGENT GLUCOSIDES IN ORGANIC SOLVENT-WATER MIXTURES USING NATIVE GLUCOSIDASES

The behaviour of native alpha-glucosidase and beta-glucosidase was stu died in reverse hydrolytic processes to compare their activity in bind ing various alcohols to glucose with the aim to synthesize detergent O -alkyl or aralkyl glucosides. In the presence of glucose as substrate glucosidases retained after 48 h incubation at room temperature about 50% of their original acitivity depending on water content and type of alcohol. It was found that native alpha- and beta-glucosidases have s ignificant O-glycosylation acitivity with glucose as substrate and wit h different alcohols serving both as reaction partners and solvents. T he yields were moderate (about 20%) because inactivation of both gluco sidases was rather fast even in the presence of glucose substrate. The best results were found at room temperature with alcohols containing 10-15% water and at reaction times not longer than 72 h.