EVALUATION OF THE PERFORMANCE OF 2D H-1 PFG ABSORPTION MODE DQ SPECTROSCOPY IN THE ANALYSIS OF CHEMICAL AND BIOLOGICAL MOLECULES

The performance of the H-1 2D PFG-DQ experiment is analysed with theor y and with some practical examples. The use of different angles for th e detection pulse allows sensitivity optimization and spectral editing . Almost complete water suppression, high sensitivity, predominantly a bsorption lineshape, very low t(1) noise and absence of strong diagona l signals make the method very suitable for the analysis of complex mi xtures, e.g. biofluids, cell cultures, natural product extracts and co mbinatorial chemistry samples. The method is also compared with the we ll known TOCSY and COSY experiments, (C) John Wiley & Sons Ltd.