Dl. Patton et al., 0.25-PERCENT CHLORHEXIDINE GLUCONATE GEL - A PROTECTIVE TOPICAL MICROBICIDE, Sexually transmitted diseases, 25(8), 1998, pp. 421-424
Background and Objectives: An estimated 4 million new cases of chlamyd
ial infection occur each year. This experiment assessed the effects of
a vaginally applied gel formulation of 0.25% chlorhexidine gluconate
on chlamydial infection and on the vaginal ecosystem, Study Design: Tw
elve monkeys were treated with a single application of 0.25% chlorhexi
dine gluconate. These animals were assessed for changes in vaginal flo
ra before and at 30 minutes, 1 day, and 2 days postapplication by micr
obiologic analysis. Cervical and vaginal tissues were assessed by colp
oscopy at each time point, Five monkeys received a single application
of 0.25% chlorhexidine gluconate gel followed (30 minutes) by a cervic
al inoculation with Chlamydia trachomatis. Four monkeys were inoculate
d with Chlamydia only, Cervicovaginal tissues were assessed via modifi
ed colposcopy, vaginal swabs were collected for assessment of vaginal
flora, and cervical swabs were collected for detection of Chlamydia (c
ulture/ligase chain reaction) at baseline and days 1, 2, and 7 postino
culation, Results: Changes in vaginal flora were minimal in all monkey
s. Application of 0.25% chlorhexidine gluconate did not affect adverse
ly vaginal colonization by lactobacilli. All chlamydial infection cont
rol monkeys were infected, whereas none of the five monkeys pretreated
with chlorhexidine gluconate were positive for C, trachomatis by cult
ure or ligase chain reaction, Colposcopic observations remained largel
y unchanged in all groups, Conclusions: A 0.25% chlorhexidine gluconat
e gel was protective against chlamydial infection in all animals teste
d, had no adverse effect on the vaginal flora, and had minimal effect
on cervicovaginal tissues after a single application.