CHEMICAL DEGRADATION KINETICS OF RECOMBINANT HIRUDIN (HV1) IN AQUEOUS-SOLUTION - EFFECT OF PH

Purpose. To gain information on the chemical stability pattern and the kinetics of the degradation of recombinant hirudin variant HV1 (rHir) , a thrombin-specific inhibitor protein of 65 amino acids. in aqueous solution as a function of pH. Methods. Stability of rHir was monitored at 50 degrees C in the framework of a classical pH-stability study in aqueous buffers pH 1-9.5. Two capillary electrophoresis (CE) protocol s were used; one for the kinetics of succinimide formation at Asp(53)- Gly(54) (C-terminal tail) and Asp(33)-Gly(34) (loop section), the othe r for the kinetics of rHir degradation. To check for potential effects of conformational changes by thermal denaturation, circular dichroism (CD) measurements were performed between 25 and 80 degrees C. Results . Throughout the pH range studied no effect of thermal denaturation on rHir confirmation at 50 degrees C was observed, rHir was most stable at a neutral pH whereas, at slightly acidic pH, an intermediate stabil ity plateau was found. Both, strongly acidic and alkaline conditions l ed to fast rHir degradation. Depending on the pH of degradation. rHir was found to degrade in Various combinations of multiple parallel and sequential degradation patterns. Special focus was on succinimide form ation at Asp(53)-Gly(54) (C-terminal tail) and Asp(33)-Gly(34) (loop) and on the potential of isoAsp formation in position 53 and 33. Conclu sions. Chemical rHir stability in the intermediate pH range depends st rongly on succinimide formation. At slightly acidic conditions succini mides represent the major degradation product (up to 40%). Around neut ral pH succinimides react further, presumably by isoAsp formation, and concentrations remain low. Relative preference of succinimide formati on in the C-terminal tail domain versus the loop domain is explained b y higher backbone flexibility in the tail.