U-74389G SUPPRESSES LIPID-PEROXIDATION AND APOPTOSIS FOLLOWING FOCAL CEREBRAL-ISCHEMIA AND REPERFUSION IN RATS

Objective To study the antioxidant and antiapoptotic effects of U-7438 9G on ischemic brain in rats. Methods Right focal cerebral ischemia an d reperfusion was induced by suturing in 103 male SD rats weighing 280 -310 g. To determine LPO in the cortex and striatum, we randomly divid ed 78 rats into 5 groups: group 1, sham-operative group (6 rats); grou p 2, control (18); group 3, U-74389G administration before ischemia (1 8); group 4, U-74389G administration before reperfusion; group 5, CS-4 vehicle as negative control. Groups 2-5 were further divided into 3 s ubgroups according to the duration of reperfusion. Another 25 rats wer e similarly grouped (5) to detect apoptosis with TUNEL stain. Results The concentrations of MDA in groups 3 and 4 were reduced and the activ ities of SOD and concentrations of GSH were partially restored as comp ared with the control group. These effects were more significant in gr oup 3 than in group 4. Otherwise, CS-4 can not provide protective effe cts. The number of apoptotic cells in groups 3 (P < 0.01) and 4 (P < 0 .05) was significantly reduced as compared with the control group. Con clusions U-74389G has antioxidant and antiapoptotic effects on ischemi c brain in rats. The administration of U-74389G before the onset of is chemia was more useful than that before the onset of reperfusion.