EVALUATION OF AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY (ELISA) FOR QUANTIFYING DDAC IN INDUSTRIAL TREATING SOLUTIONS

An enzyme-linked immunosorbent assay (ELISA), based on polyclonal anti bodies, was used to quantify didecyldimethylammonium chloride (DDAC) i n industrial antisapstain treating solutions. The initial ELISA valida tion included: cross-reactivity with similar analytes, interference wi th non-related compounds, percentage of false negative and false posit ive determinations, and comparison of the immunoassay data with the da ta from titration and HPLC methods. ELISA and the titration method gav e comparable results. However, higher DDAC values were obtained for in dustrial treating solutions measured by ELISA than by HPLC. Immunoassa ys may be a potential analytical tool for the quality control program of DDAC type antisapstain formulations, if specificity could be improv ed.