IDENTIFICATION OF NOVEL GENES THAT ARE DIFFERENTIALLY EXPRESSED IN HUMAN COLORECTAL-CARCINOMA

By using mRNA differential display technology, we have identified thre e cDNA clones, myl 3, myl 4, and myl 6, to show significant changes in expression between human colorectal tumor and paired normal tissue. N orthern blot analysis indicated that clone myl 3 expression was elevat ed in normal tissue, and clone myl 4 expression was elevated in tumor tissue. Nucleotide sequence analysis revealed that clones myl 3 and my l 4 have not been previously identified. However, clone myl 6 appears to be the human homolog of the 3' end region of tissue inhibitor of me talloproteinase 3 (TIMP-3). Northern blot analysis showed that a 2.5 k b TIMP-3 transcript was expressed at a much higher level in normal tis sue than the colorectal tumor. (C) 1998 Elsevier Science B.V. All righ ts reserved.