IN-SITU MONITORING OF ADSORPTION OF LYSOZYME ONTO QUARTZ USING AN ELECTRODE-SEPARATED PIEZOELECTRIC SENSOR

The adsorption of lysozyme from phosphate-buffered saline (pH 7.0) ont o a quartz crystal interface was investigated in situ using an electro de-separated piezoelectric sensor (ESPS). Increasing amounts of lysozy me adsorbed onto a quartz crystal surface resulted in linearly decreas ing oscillating frequencies of the ESPS. The lysozyme adsorbed is part ially desorbed after rinsing with the phosphate-buffered saline. The a mounts adsorbed reversibly and irreversibly were estimated. The influe nce of the surface roughness of the quartz crystal on the adsorption d ensity measurement is discussed. (C) 1998 Academic Press.