IRRADIATION PRETREATMENT BEFORE FIBROBLAST IMPLANTATION IN EXPERIMENTAL PVR

Fibroblast injection into the vitreous body causes traction detachment in the rabbit's eye. Various working groups reported different result s on the main causes of the development of experimental PVR. These con tradictions encouraged us to investigate the main source of experiment al PVR by irradiating the ocular tissues before fibroblast implantatio n thus suppressing cell proliferation originating from host tissue. Ov er a period of 3 weeks, 22 eyes received ten radiations in a total dos age of 3000 rad. After the last radiation, 250,000 fibroblasts were im planted into 22 eyes. In another 4 eyes, fibroblast implantation but n o radiation was carried out. After 8 weeks, 59% of 22 eyes developed d ifferent stages of retinal detachment. Comparison with the group of un irradiated eyes, which developed retinal detachment in 85%, revealed n o significant differences in the number of detachments.