GENERAL-EDUCATION FOR PEAK-CAPACITY IN COLUMN CHROMATOGRAPHY

In this study, the peak capacities for packed capillaries used in vari ous forms of chromatography were investigated, Experiments found that the peak width at half-height (w(1/2)) linearly increased with increas ing retention time when operating under isothermal, isobaric, and isoc ratic conditions in capillary gas chromatography, solvating gas chroma tography, supercritical fluid chromatography, and liquid chromatograph y, From this relationship, a general peak capacity (n) expression was obtained. This expression covers conventional packed, packed capillary , and open tubular column chromatographies, With this general expressi on, it is possible to directly compare the separating capabilities of the various column types and chromatographic techniques.