TISSUE-SPECIFIC DNA METHYLATION IN HAEMANTHUS-KATHARINAE BAK. (AMARYLLIDACEAE)

Authors
SAKOWICZ T OLSZEWSKA MJ LUCHNIAK P KAZMIERCZAK J
Citation
T. Sakowicz et al., TISSUE-SPECIFIC DNA METHYLATION IN HAEMANTHUS-KATHARINAE BAK. (AMARYLLIDACEAE), Acta Societatis Botanicorum Poloniae, 67(2), 1998, pp. 175-180
Citations number
31
Categorie Soggetti
Plant Sciences
Journal title
Acta Societatis Botanicorum Poloniae → ACNP
ISSN journal
00016977
Volume
67
Issue
2
Year of publication
1998
Pages
175 - 180
Database
ISI
SICI code
0001-6977(1998)67:2<175:TDMIHB>2.0.ZU;2-V
Abstract
The level of DNA methylation was compared in root meristem, adult leaf and endosperm of monocotyledonous species, Haemanthus katharnae, with the use of HPLC, restriction analysis, Southern blot hybridization an d in situ nick-translation driven by restriction enzyme HhaI. The high est level of 5-methylcytosine was observed in adult leaf whose nuclear chromatin is particularly condensed, and the lowest in endosperm. The level of DNA methylation of repetitive HaeIII 400bp sequence follows that of the total DNA.