MOLECULAR CYTOGENETIC ANALYSIS OF A NEW WHEAT-RYE HYBRID WITH C-BANDING AND GENOMIC IN-SITU HYBRIDIZATION (GISH)

The hexaploid winter wheat (Triticum aestivum L.) line Martonvasari 9 kr1 (Mv9kr1) possessing recessive crossability alleles (kr(1)kr(1)kr(2 )kr(2)) was pollinated with rye (Secale cereale L. cv. Lovaszpatonai). The mean crossability percentage was fairly high (68.4%). The chromos ome number distribution was examined in mitotic chromosome spreads of octoploid triticale obtained via colchicine treatment of the initial h ybrid and it was found to range from 51 to 56. Eighteen amphiploid (C- 1) plants were backcrossed with Mv9kr1 for the purpose of producing ad dition lines and subsequent alien gene transfer into wheat. All the ry e (Secale cereale L. cv. Lovaszpatonai) chromosomes were identified in mitotic chromosome spreads of octoploid triticale with the help of C- banding and fluorescence in situ hybridization (FISH).