PURIFICATION, CRYSTALLIZATION AND PRELIMINARY CRYSTALLOGRAPHIC ANALYSIS OF BOVINE CYTOSOLIC BRAIN-TYPE CREATINE-KINASE

Authors
LOEW A BAX B
Citation
A. Loew et B. Bax, PURIFICATION, CRYSTALLIZATION AND PRELIMINARY CRYSTALLOGRAPHIC ANALYSIS OF BOVINE CYTOSOLIC BRAIN-TYPE CREATINE-KINASE, Acta crystallographica. Section D, Biological crystallography, 54, 1998, pp. 989-990
Citations number
9
Categorie Soggetti
Crystallography,"Biochemical Research Methods",Biophysics,Biology
Journal title
Acta crystallographica. Section D, Biological crystallography → ACNP
ISSN journal
09074449
Volume
54
Year of publication
1998
Part
5
Pages
989 - 990
Database
ISI
SICI code
0907-4449(1998)54:<989:PCAPCA>2.0.ZU;2-4
Abstract
Creatine kinase (E.C. 2.3.7.2) is an important enzyme in energy metabo lism which catalyzes the reversible transfer of a phosphoryl group bet ween phosphocreatine and ADP to give ATP. Large quantities of a brain- type creatine kinase have been isolated from bovine photoreceptor cell s and crystals suitable for X-ray diffraction analysis have been obtai ned by hanging-drop vapor diffusion. Crystals grow as tetragonal bipyr amids in space group P4(3)2(1)2 with cell dimensions a = b = 96.49, c = 108.42 Angstrom and diffract to at least 2.7 Angstrom resolution.