U. Gerike et al., PRELIMINARY CRYSTALLOGRAPHIC STUDIES OF CITRATE SYNTHASE FROM AN ANTARCTIC PSYCHROTOLERANT BACTERIUM, Acta crystallographica. Section D, Biological crystallography, 54, 1998, pp. 1012-1013
Citations number
13
Categorie Soggetti
Crystallography,"Biochemical Research Methods",Biophysics,Biology
Recombinant citrate synthase from a psychrotolerant bacterium, DS2-3R,
recently isolated in Antarctica, has been crystallized. The crystals
belong to space group P6(1)22 or P6(5)22, with cell dimensions a = b =
70.8, c = 307.8 Angstrom. Diffraction data collected on a synchrotron
from a cryoprotected crystal extend to at least 2.0 Angstrom. Knowled
ge of the structure of this enzyme Rill add to the understanding of to
ld activity and thermolability and will be of biotechnological interes
t. Previously, the structure of citrate synthase from Archaea inhabiti
ng environments at 328 and 373 K, has been reported. This present stud
y will extend our understanding of the structural integrity and activi
ty of proteins at the temperature extremes of life.