EXPRESSION, PURIFICATION, CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF CYCLOPHILIN-A FROM THE BOVINE PARASITE TRYPANOSOMA-BRUCEI-BRUCEI

Authors
DAOTHI MH TRANSUE TR PELLE R MURPHY NB POORTMANS F STEYAERT J
Citation
Mh. Daothi et al., EXPRESSION, PURIFICATION, CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF CYCLOPHILIN-A FROM THE BOVINE PARASITE TRYPANOSOMA-BRUCEI-BRUCEI, Acta crystallographica. Section D, Biological crystallography, 54, 1998, pp. 1046-1048
Citations number
14
Categorie Soggetti
Crystallography,"Biochemical Research Methods",Biophysics,Biology
Journal title
Acta crystallographica. Section D, Biological crystallography → ACNP
ISSN journal
09074449
Volume
54
Year of publication
1998
Part
5
Pages
1046 - 1048
Database
ISI
SICI code
0907-4449(1998)54:<1046:EPCAPA>2.0.ZU;2-O
Abstract
Cyclophilin A from the bovine parasite Trypanosoma brucei brucei has b een cloned, expressed in Escherichia coli, purified and crystallized i n the presence of cyclosporin A using ammonium sulfate as a precipitan t. The crystals belong to the orthorhombic crystal system with unit-ce ll dimensions of a = 118.61, b = 210.15 and c = 153.21 Angstrom. A dat a set complete to 2.7 Angstrom has been collected using rotating-anode radiation, however the crystals diffract to at least 2.1 Angstrom res olution using synchrotron radiation.