CRYSTALLIZATION AND PRELIMINARY-X-RAY STUDIES OF PURINE NUCLEOSIDE PHOSPHORYLASE FROM CELLULOMONAS SP

Authors
BZOWSKA A TEBBE J LUIC M WIELGUSKUTROWSKA B SCHRODER W SHUGAR D SAENGER W KOELLNER G
Citation
A. Bzowska et al., CRYSTALLIZATION AND PRELIMINARY-X-RAY STUDIES OF PURINE NUCLEOSIDE PHOSPHORYLASE FROM CELLULOMONAS SP, Acta crystallographica. Section D, Biological crystallography, 54, 1998, pp. 1061-1063
Citations number
37
Categorie Soggetti
Crystallography,"Biochemical Research Methods",Biophysics,Biology
Journal title
Acta crystallographica. Section D, Biological crystallography → ACNP
ISSN journal
09074449
Volume
54
Year of publication
1998
Part
5
Pages
1061 - 1063
Database
ISI
SICI code
0907-4449(1998)54:<1061:CAPSOP>2.0.ZU;2-X
Abstract
The commercially available enzyme purine nucleoside phosphorylase (PNP ) from Cellulomonas sp. was purified by ion-exchange chromatography, p artially sequenced and crystallized in two different crystal forms usi ng the hanging-drop vapour-diffusion technique. Crystal form A grows a s polyeders and/or cubes in the cubic space group P4(2)32 with unit-ce ll dimension a = 162.5 Angstrom. Crystal form B appears as thick plate s in the space group P2(1)2(1)2(1) with unit-cell dimensions a = 63.2, b = 108.3 and c = 117.4 Angstrom. Both crystal forms contain three mo nomers (one trimer) in the asymmetric unit.