IDENTIFICATION OF SHYC, A NOVEL GENE EXPRESSED IN THE MURINE DEVELOPING AND ADULT NERVOUS-SYSTEM

The embryonal carcinoma cell line P19 responds to treatment with retin oid acid by differentiation into neuronal cell types [2], Using radioa ctively labeled cDNA derived from differentiating P19 cells we screene d an adult mouse brain cDNA library and isolated a gene named shyc for selective hybridizing clone. The encoded protein did not reveal homol ogy to any known protein. We used in situ hybridization on mouse embry onic and adult brain sections to study shyc expression. The developing and embryonic nervous system showed the most prominent hybridization signals. In the adult brain the olfactory pathway was marked by shyc e xpression, (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.