DIFFERENTIAL EXPRESSION OF TOMATO (LYCOPERSICON-ESCULENTUM L) GENES ENCODING SHIKIMATE PATHWAY ISOENZYMES .2. CHORISMATE SYNTHASE

In tomato (Lycopersicon esculentum L. cv. UC82b) two distinct genes (d esignated LeCS1 and LeCS2) code for chorismate synthase. The correspon ding cDNAs have been isolated and characterized. The deduced amino aci d sequences are 88% identical. Both genes encode chorismate synthases with putative plastid-specific N-terminal transit peptides. The two ge nes are predominantly expressed in flowers and roots and, to a lesser extent, in stems, leaves, and cotyledons, but the steady-state levels of LeCS1-specific transcripts are consistently higher than those of th e LeCS2-specific transcripts.